A.I. Fedoriv

Condition of immunity in patients with chronic nonbacterial prostatitis conditioned herpesvirus infection

The aim of the study was to study the state of local and systemic immunity in patients with chronic abacterial prostatitis caused by herpes virus infection.

500 men aged from 30 to 50 years old were examined, who after general urological examinations diagnosed with chronic nonbacterial prostatitis in all patients. For the viral etiology of chronic nonbacterial prostatitis, the presence of the following criteria was taken into account: the presence of high-avid IgG against herpes and cytomegalovirus, the absence of acute phase Ig M against herpes and cytomegalovirus, a history of genital herpes, and also the main criterion - the presence of viral DNA in prostate secretion/ejaculate PCR. The state of local and systemic immunity was investigated by determining the serum IFN-g and the content of IgA in the prostate secretion/semen of patients. To confirm the presence of inflammation in the prostate, the levels of proinflammatory interleukin 6 (IL-6) and antiinflammatory interleukin 10 (IL-10) were determined in the ejaculate patients. According to the results of the seropositive examination, 470 patients (94%) were identified. Of these, 63 (13,4%) with viral abacterial prostatitis were targeted. The 1st group of patients consisted of 35 patients in whom DNA of the herpes simplex virus (HSV) was detected. 2nd group - 15 patients, in the ejaculate/secret of which the cytomegalovirus (CMV) gene was detected. The 3rd group consisted of 13 patients in whom the DNA of HSV and TsMV was detected. The 4th group of patients - the control group, 20 healthy men of the same age. According to the results obtained in the control group, the level of IFN-g was 23,6±0,8 pg/ml. Accordingly, in group 1 the level of IFN-g was contained within 17,6±0,3 pg/ml (P<0,05), in group 2 - 16,8±0,2 pg/ml (P <0,05), in group 3 - 15,8±0.4 pg/ml (P <0,05). In the study of the level of secretory immunoglobulin A (sIGA), the following results were obtained: the control group was 35,6±0,7 mg/l, in group 1 the level of sIgA was 20,5±0,4 mg/l (P<0.05), in the second - 19.5±0.3 mg/l (P<0,05), in the third, respectively 16,5±0,3 mg/l, (P<0.05). Regarding cytokine status in patients with lesions of the prostate herpes virus, reliable elevated levels of pro-inflammatory IL-6 were obtained in all groups compared to the control group, as well as a decrease in the level of anti-inflammatory IL-10, respectively. In patients with chronic abacterial prostatitis due to herpes virus infection, there are significant changes in the immune status of both systemic and local immunity. High rates of immunosuppression were found in patients with prostate disease with a combination of herpes simplex viruses and cytomegalovirus.